Autoantibodies against MAG, myelin, GAD

Neurology Mosaic 1

  • Screening test for the detection of antibodies against neuronal antigens.
  • Indications: neurological diseases.
  • Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
  • Substrates: cerebellum (monkey), nerves (monkey), intestine (monkey)
  • Primate cerebellum and primate nerves are the standard substrates for the determination of various neuronal antibodies. The parallel use of primate intestine permits the reliable differentiation from other autoantibodies (e.g. ANA) and makes it possible to distinguish between anti-Ri and anti-Hu.
  • Antibodies against grey matter react intensively with the stratum granulosum and in a weaker form with the stratum moleculare of the cerebellum. Target antigen: glutamic acid decarboxylase (GAD). Clinical significance: stiff person syndrome, diabetes mellitus type I.
  • Antibodies against Yo stain exclusively the cytoplasm of the Purkinje cells in the cerebellum. Clinical significance: paraneoplastic neurological syndromes (PNS), indication of a malignoma.
  • In the case of antibodies against Hu and Ri all neurone nuclei in the grey matter show a granular fluorescence. Hu antibodies react in the intestine with cell nuclei of the plexus myentericus, whereas Ri antibodies do not. Clinical significance: paraneoplastic neurological syndromes (PNS), indication of a malignoma.
  • The white matter of the cerebellum is stained by antibodies against myelin, which present as hyaline cylinders in tissue sections of peripheral nerves. A "droplike" ring-shaped fluorescence is observed in cross sections of nerves.
  • Antibodies against myelin-associated glycoprotein (MAG), on the other hand, show a streaky fluorescence pattern on nerve tissue and a mostly fine-granular ring-shaped fluorescence on cross sections of peripheral nerves. Clinical significance: paraproteinaemic neuropathy.

ProductOrder number
Neurology Mosaic 1 FA 1111-1