ChLIA

EUROIMMUN ChLIA

Random access and highest speed

Advantages of EUROIMMUN ChLIA

  • Fully automated processing from primary sample to result using the IDS-iSYS Multi-Discipline Automated System or the IDS-i10
  • Highestspeed – results in just 30 minutes
  • Ready-for-use reagent cartridges and calibrators with long stability, also cooled in the instrument
  • Transmission of quality control data for error-free and quick loading with little manual effort
  • Quantification based on calibration curves which are stable over several weeks
  • Broad dynamic analytical range due to high signal intensity at high sensitivity and specificity

  • Test principle

    Chemiluminescence immunoassays (ChLIA) from EUROIMMUN use magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals. Performance and evaluation are fully automated with the IDS-iSYS Multi-Discipline Automated System or the IDS-i10.


      Antibody detection by ChLIA

      The antigen-coated magnetic particles are incubated with diluted patient samples. If a sample contains specific antibodies directed against the antigen, these bind to the antigen-coated magnetic particles. In the next step, acridinium-labelled antibody (conjugate) is added. This binds to the specific antibodies.  A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antibody concentration in the patient sample within the measurement range and is automatically converted into a concentration by the instrument.


      Antigen detection by ChLIA

      The antibody-coated magnetic particles are incubated with the patient sample and an antigen-specific biotinylated antibody. During the incubation, the antigen is bound by the magnetic particle-coupled antibody as well as by the biotinylated antibody. In the next step, acridinium-labelled ExtrAvidin (conjugate) is added. This binds to the biotinylated antibody. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antigen concentration in the patient sample within the indicated measurement range and is converted automatically into a concentration by the instrument.


    • Automation

      IDS-i10 and IDS-iSYS

      Product

    Techniques

    Immunoblot

    Technique

    Microarray

    Technique

    Real-Time PCR

    Technique

    Radioimmunoassays

    Technique

    Dried Blood Spots (DBS)

    Technique
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