ChLIA

EUROIMMUN ChLIA

Random access and highest speed

Advantages of EUROIMMUN ChLIA

  • Fully automated processing from primary sample to result using the RA Analyzer 10, also as part of a laboratory track system
  • Highestspeed – results in just 30 minutes
  • Ready-for-use reagent cartridges and calibrators with long stability, also cooled in the instrument
  • Automated transmission of quality control data by RFID chip for error-free and quick loading with little manual effort
  • Quantification based on calibration curves which are stable over several weeks
  • Broad dynamic analytical range due to high signal intensity at high sensitivity and specificity

  • Test principle

    Chemiluminescence immunoassays (ChLIA) from EUROIMMUN use magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals. Performance and evaluation are fully automated with the RA Analyzer 10.


      Antibody detection by ChLIA

      The antigen-coated magnetic particles are incubated with diluted patient samples. If a sample contains specific antibodies directed against the antigen, these bind to the antigen-coated magnetic particles. In the next step, acridinium-labelled antibody (conjugate) is added. This binds to the specific antibodies.  A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antibody concentration in the patient sample within the measurement range and is automatically converted into a concentration by the instrument.


      Antigen detection by ChLIA

      The antibody-coated magnetic particles are incubated with the patient sample and an antigen-specific biotinylated antibody. During the incubation, the antigen is bound by the magnetic particle-coupled antibody as well as by the biotinylated antibody. In the next step, acridinium-labelled ExtrAvidin (conjugate) is added. This binds to the biotinylated antibody. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antigen concentration in the patient sample within the indicated measurement range and is converted automatically into a concentration by the instrument.


    • Automation

      RA Analyzer 10

      Product

    Techniques

    Immunoblot

    Technique

    Microarray

    Technique

    Real-Time PCR

    Technique

    Radioimmunoassays

    Technique
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