Paraneoplastic neurological syndromes

Clinical information

Paraneoplastic neurological syndromes (PNS) are diseases of the central and peripheral nervous system that occur in direct relation to tumour development. However, these are not caused directly by the tumour, by its metastases or by any side-effects from therapy using cytostatic drugs or radiation treatment. PNS occur in approximately 15% of malignant diseases, particularly in lung tumours.

Depending on the type of tumour, tumour cells express antigens, e.g. amphiphysin, CV2/CRMP5, PNMA2 (Ma2/ Ta), Ri, Yo, Hu, ZIC4 or Tr (DNER) which can induce the formation of specific autoantibodies. These autoantibodies bind to the respective antigens localised in the nervous tissue and can thus cause neurological disorders.

In the literature two types of nomenclature are used for PNS-specific autoantibodies. One is based on the first two letters of the index patient's name (e.g. Hu for Hull, Yo for Young, Ma for Margret), the other on the initial letters of the immunohistochemical staining (ANNA = anti-nuclear neuronal antibodies). We use the nomenclature of Posner (anti-Hu, -Yo, -Ma etc.), since this is antigen-based and independent of the test procedure.

Diagnostics

The European network for paraneoplastic neurological diseases (PNS Euronetwork) has published diagnostic criteria. These lead to two levels of diagnostic certitude, namely a definitive or a possible paraneoplastic syndrome. In serological diagnostics, autoantibodies in PNS should always be determined using two unrelated methods. Various line blots (EUROLINE) are available in addition to indirect immunofluorescence tests with special BIOCHIP Mosaics for neurology. Thus, test results can be compared and, if necessary, confirmed. Results should only be used for diagnosis when both test results are congruent in qualitative determination and are in line with the clinical symptoms.

Selected Products

Method
Parameter
Substrate
Species/ Antigen
IIFT
brain: grey and white matter,
Purkinje cell cytoplasm (Yo),
Hu and Ri antigen
CV2, Ma, amphiphysin
cerebellum
monkey
EUROLINE
positive control serum: IgG, human, 100x concentrated
for DL 1111-1 G, DL 1111-2 G, DL 1111-4 G and DL 1111-7 G
IIFT
Neurology Mosaic 1 EUROPattern
Yo, Hu, Ri, CV2, Ma, amphiphysin
medullated nerves
non-medullated nerves
3 BIOCHIPs per field:
cerebellum
nerves
intestinal tissue

monkey
monkey
monkey
IIFT
Neurology Mosaic 1
Yo, Hu, Ri, CV2, Ma, amphiphysin
medullated nerves
non-medullated nerves
3 BIOCHIPs per field:
cerebellum
nerves
intestinal tissue

monkey
monkey
monkey
IIFT
Neurology Mosaic14
cerebellum antigens
non-medullated nerves
2 BIOCHIPs per field:
cerebellum
non-medullated nerves

monkey
monkey
IIFT
Yo, Hu, Ri, CV2, Ma, amphiphysin
medullated nerves
non-medullated nerves
cell nuclei (ANA)
cerebellum
nerves
intestinal tissue
HEp-2 cells
(4 BIOCHIPs per field)
monkey
monkey
monkey
human
IIFT
Neurology Mosaic 17
Yo, Hu, Ri, CV2, Ma, amphiphysin, NMO
cerebrum antigens, NMO
NMO, optic nerve antigens
aquaporin-4
5 BIOCHIPs per field
cerebellum
cerebrum
optic nerve
transfected cells
control transfection

monkey
monkey
monkey
EU 90
EU 90
IIFT
Neurology Mosaic 2
Yo, Hu, Ri, CV2, Ma, amphiphysin
medullated nerves
2 BIOCHIPs per field:
cerebellum
nerves

monkey
monkey
EUROLINE
Neuronal Antigens Profile 2
(amphiphysin, CV2, PNMA2
(Ma-2/Ta), Ri, Yo, Hu separately)
EUROLINE
EUROLINE
Neuronal Antigens Profile PLUS RST
(amphiphysin, CV2, PNMA2
(Ma-2/Ta), Ri, Yo, Hu,
recoverin, SOX1, titin separately)
EUROLINE
EUROLINE
Neuronal Antigens Profile
SOX1, titin
EUROLINE
EUROLINE
positive control serum: IgG, human, 100x concentrated
for DL 1111-6 G
EUROLINE
Paraneoplastic Neurologic Syndromes - 12 Ag
(amphiphysin, CV2, PNMA2 (Ma-2/Ta), Ri, Yo, Hu,
recoverin, SOX1, titin, Zic4, GAD65, Tr (DNER) separately)
EUROLINE
IIFT
Neurology Mosaic 8 EUROPattern
Yo, Hu, Ri, CV2, Ma, amphiphysin
medullated nerves
non-medullated nerves
pancreas islets
4 BIOCHIPs per field:
cerebellum
nerves
intestinal tissue
pancreas

monkey
monkey
monkey
monkey
IIFT
Neurology Mosaic 8
Yo, Hu, Ri, CV2, Ma, amphiphysin
medullated nerves
non-medullated nerves
pancreas islets
4 BIOCHIPs per field
cerebellum
nerves
intestinal tissue
pancreas

monkey
monkey
monkey
monkey
IIFT
antibodies against Delta and Notch-like epidermal growth factor-related receptor
(DNER CBA control)
IIFT
Delta and Notch-like epidermal
growth factor-related receptor (DNER)
transfected cells
control transfection
(2 BIOCHIPs per field)
EU 90
EU 90
IIFT
antibodies against Purkinje cell cytoplasm
(Yo ab control)
IIFT
Purkinje Cell Mosaic 1
Yo/CDR2
DNER
ITPR1
CARP
4 BIOCHIPs per field:
transfected cells
transfected cells
transfected cells
transfected cells

EU 90
EU 90
EU 90
EU 90
IIFT
antibodies against neuronal nuclei: Ri antigen
(Ri ab control)
IIFT
antibodies against neuronal nuclei: Hu antigen
(Hu ab control)
IIFT
zinc finger protein ZIC4
transfected cells
control transfection
(2 BIOCHIPs per field)
EU 90
EU 90
Back to top
Contact person on site
Please click on a country or enter yours
North America
South & Central America
Europe
Asia
Oceania
Middle East & Africa