Autoantibodies against granulocyte cytoplasm

Granulocyte Mosaic 2

Granulocytes (HCHO) top and granulocytes (EOH) bottom
  • Screening test for the detection of antibodies against granulocyte cytoplasm (ANCA).
  • Indications: primary sclerosing cholangitis, ulcerative colitis, Crohn's disease, Wegener‘s granulomatosis, various forms of glomerulonephritis.
  • Initial dilution: serum undiluted and 1 : 10 in parallel; conjugate class anti-human IgG, FITC-labelled.
  • Using ethanol-fixed granulocytes, antibodies against granulocyte cytoplasm can be detected. In this case, two fluorescence patterns have to be differentiated: a granular fluorescence which is distributed evenly over the entire cytoplasm, leaving the cell nuclei free (cytoplasmic type, cANCA) or a smooth fluorescence wrapped ribbon-like around the cell nuclei (perinuclear type, pANCA).
  • Antibodies against all relevant granulocyte antigens as well as against further, as yet unknown antigens are detected simultaneously.
ProductOrder number
Granulocyte Mosaic 2FA 1201-2