Thyroid-specific autoantibodies

Thyroid gland, unfixed and thyroglobulin BIOCHIP: antibodies against thyroid microsomes and thyroglobulin

Detection of antibodies against thyroid gland antigens.
Indications: Graves' disease, Hashimoto's autoimmune thyroiditis.
Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
Using unfixed thyroid tissue, two important thyroid-specific antibodies can be found: Autoantibodies against thyroid microsomes (MAb) give a granular staining in the cytoplasm of the follicle epithelium (target antigen: thyroid peroxidase, TPO). Autoantibodies against thyroglobulin (TAb) react with the colloid of all follicles of the thyroid tissue and cause a reticular fluorescence pattern.
With the thyroglobulin-coated BIOCHIP, autoantibodies against thyroglobulin (TG) can be confirmed monospecifically in one and the same test procedure.
This BIOCHIP Mosaic can be supplemented as required with further substrates, e.g. rat kidney, to achieve a differentiation of antibodies against thyroid microsomes from mitochondrial antibodies (AMA). For a serological diagnosis of autoimmune polyendocrinopathies, BIOCHIPs with frozen sections of pancreas, adrenal gland, ovary, testis and stomach can be added.

Product	Order number
EUROPLUS thyroid gland (monkey) and thyroglobulin antigen	FA 1010-3

Incubated anti-thyroid antigen ELISAs

Monospecific determination of antibodies against thyroglobulin (TG), thyroid peroxidase (TPO), and thyrotropin receptor (TSH-R).
Indications: Graves' disease, Hashimoto's autoimmune thyroiditis.
Serum dilution 1 : 201 (exception: anti-TSH-R); conjugate class anti-human IgG, POD-labelled (anti-TSH-R: avidin-labelled).
3-point calibration (exception: TSH-R, 5-point calibration).
The quantification is carried out according to international reference preparations (anti-TG: NIBSC 65/93; anti-TPO: NIBSC 66/387; anti-TSH-R: NIBSC 90/672).
Thyroglobulin/TSH-R: native antigen; thyroid peroxidase: recombinant antigen.

Product	Order number
Anti-Thyroid Peroxidase ELISA	EA 1012 G
Anti-Thyroglobulin ELISA	EA 1013 G
Anti-TSH Receptor ELISA	EA 1015 G
Anti-TSH Receptor Fast ELISA	EA 1015-1 G

RIA for the thyroid diagnostic

Monospecific detection of autoantibodies against thyroglobulin (TG), thyroid peroxidase (TPO) and thyrotropin receptor (TSH-R).
Indications: Graves' disease, Hashimoto's thyroiditis.
Serum dilutions: 1:51 for anti-TG and anti-TPO (magnetic), 1:21 for anti-TPO (precipitation), undiluted for all remaining test kits.
5-point to 6-point calibration (quantitative).