Thyroid-specific autoantibodies
Thyroid gland, unfixed and thyroglobulin BIOCHIP: antibodies against thyroid microsomes and thyroglobulin |
- Detection of antibodies against thyroid gland antigens.
- Indications: Graves' disease, Hashimoto's autoimmune thyroiditis.
- Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
- Using unfixed thyroid tissue, two important thyroid-specific antibodies can be found: Autoantibodies against thyroid microsomes (MAb) give a granular staining in the cytoplasm of the follicle epithelium (target antigen: thyroid peroxidase, TPO). Autoantibodies against thyroglobulin (TAb) react with the colloid of all follicles of the thyroid tissue and cause a reticular fluorescence pattern.
- With the thyroglobulin-coated BIOCHIP, autoantibodies against thyroglobulin (TG) can be confirmed monospecifically in one and the same test procedure.
- This BIOCHIP Mosaic can be supplemented as required with further substrates, e.g. rat kidney, to achieve a differentiation of antibodies against thyroid microsomes from mitochondrial antibodies (AMA). For a serological diagnosis of autoimmune polyendocrinopathies, BIOCHIPs with frozen sections of pancreas, adrenal gland, ovary, testis and stomach can be added.
Product | Order number |
EUROPLUS thyroid gland (monkey) and thyroglobulin antigen | FA 1010-3 |
Incubated anti-thyroid antigen ELISAs |
- Monospecific determination of antibodies against thyroglobulin (TG), thyroid peroxidase (TPO), and thyrotropin receptor (TSH-R).
- Indications: Graves' disease, Hashimoto's autoimmune thyroiditis.
- Serum dilution 1 : 201 (exception: anti-TSH-R); conjugate class anti-human IgG, POD-labelled (anti-TSH-R: avidin-labelled).
- 3-point calibration (exception: TSH-R, 5-point calibration).
- The quantification is carried out according to international reference preparations (anti-TG: NIBSC 65/93; anti-TPO: NIBSC 66/387; anti-TSH-R: NIBSC 90/672).
- Thyroglobulin/TSH-R: native antigen; thyroid peroxidase: recombinant antigen.
Product | Order number |
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Anti-Thyroid Peroxidase ELISA | EA 1012 G |
Anti-Thyroglobulin ELISA | EA 1013 G |
Anti-TSH Receptor ELISA | EA 1015 G |
Anti-TSH Receptor Fast ELISA | EA 1015-1 G |
RIA for the thyroid diagnostic |
- Monospecific detection of autoantibodies against thyroglobulin (TG), thyroid peroxidase (TPO) and thyrotropin receptor (TSH-R).
- Indications: Graves' disease, Hashimoto's thyroiditis.
- Serum dilutions: 1:51 for anti-TG and anti-TPO (magnetic), 1:21 for anti-TPO (precipitation), undiluted for all remaining test kits.
- 5-point to 6-point calibration (quantitative).
Product | Order number |
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Anti-TPO RIA | RA 1012 |
Anti-TG RIA | RA 1013 |
Anti-TSH Receptor RIA | RA 1015 |