Autoantibodies against glomerular basement membrane (GBM)

EUROPLUS kidney (monkey) and GBM antigen

Primate kidney and GBM BIOCHIP: antibodies against glomerular basement membrane
  • Detection of antibodies against glomerular basement membrane (GBM).
  • Indications: Goodpasture's syndrome, glomerulonephritis.
  • Initial dilution 1:10,  conjugate class anti-human IgG, FITC-labelled.
  • The primary target antigen of autoantibodies in all anti-GBM glomerulonephritides, including classic Goodpasture's syndrome, is the NC1 region of the alpha-3 chain of the network-structured type IV collagen of the basement membrane lamina densa.
  • These autoantibodies can be directed against the alveolar basement membrane as well as against the glomerular basement membrane. Antibodies against the alveolar basement membrane are detected using frozen sections of primate lung (order number FA 1271-1).
  • Positive test results can be confirmed in the same test run using specific GBM BIOCHIPs which are coated with recombinant GBM antigen.

ProductOrder number
Kidney (monkey) BIOCHIPsFA 1250
EUROPLUS kidney (monkey) and GBM antigenFA 1250-1

Anti-GBM

Incubated Anti-GBM ELISA
  • Monospecific detection of antibodies against antigens of the glomerular basement membrane (GBM antigens).
  • Indication: Goodpasture's syndrome.
  • Serum dilution 1:101, conjugate class anti-human IgG, POD-labelled.
  • 3-point calibration, quantitative.
  • Antigen: alpha-3 chain of type IV collagen isolated from bovine kidney.
  • The primary target antigen of autoantibodies in all anti-GBM glomerulonephritides is the NC1 region of the alpha-3 chain of the network-structured type IV collagen of the basement membrane lamina densa.
  • The clinical disease course correlates with the antibody concentration. High titers of circulating antibodies indicate an unfavourable prognosis.
ProductOrder number
Anti-GBM ELISA EA 1251 G

EUROLINE: MPO/PR3/GBM Profile

Incubated EUROLINE MPO/PR3/GBM Profile
  • Determination of autoantibodies against antigens of neutrophil granulocytes and the glomerular basement membrane for the serological diagnosis of Wegener's granulomatosis, microscopic polyangiitis, rapid progressive glomerulonephritis, Goodpasture syndrome and other forms of vasculitis.
  • Membrane chips printed with individual lines of purified and biochemically characterised antigens. Each antigen is coated onto a separate membrance chip, providing optimal efficiency of antibody detection for each protein.
  • Serum dilution 1 : 101; conjugate class anti-human IgG, alkaline phosphatase-labelled.
  • The EUROLINE Profile allows the determination of 3 autoantibodies: antibodies against MPO, PR3 and GBM.
  • The test strips can be automatically incubated and evaluated using the EUROBlotMaster or EUROBlotOne systems and the EUROLineScan programme.

ProductOrder number
EUROLINE MPO/PR3/GBM ProfileDL 1200-3 G