Immunoblot

EUROLINE: ANA Profile 3

Incubated EUROLINE ANA Profile 3
  • Differentiation of antibodies against cell nuclei (ANA).
  • Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren’s syndrome, systemic sclerosis, poly/dermatomyositis, PBC.
  • Serum dilution 1 : 101; conjugate class anti-human IgG, AP-labelled.
  • With the EUROLINE ANA Profile 3, fifteen autoantibodies can be determined: antibodies against nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70, PM-Scl, Jo-1, centromere protein B, PCNA, dsDNA, nucleosomes, histones, ribosomal P-proteins, AMA M2.
  • Antibodies against SS-A are characteristic markers for SLE and Sjögren’s syndrome. In contrast, antibodies against Ro-52 also occur in patients with other autoimmune diseases.
  • Native antigens, purified by affinity chromatography (exception: centromere protein B, PM-Scl, Ro-52, PCNA).
  • Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster and EUROLineScan.
ProductOrder number
EUROLINE ANA Profile 3 DL 1590-3 G

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EUROLINE: Autoimmune Inflammatory Myopathies 16 Ag

Incubated EUROLINE Autoimmune Inflammatory Myopathies 16 Ag
Incubated EUROLINE Autoimmune Inflammatory Myopathies 16 Ag
  • Differentiation of myositis-associated antibodies against cell-nuclear and cytoplasmic antigens.
  • Indications: poly/dermatomyositis.
  • Serum dilution 1 : 101, conjugate class anti-human IgG, AP-labelled.
  • With the EUROLINE Autoimmune Inflammatory Myopathies 16 Ag, 16 autoantibodies can be determined: antibodies against Mi-2 alpha, Mi-2 beta, TIF1g, MDA5, NXP2, SAE1, Ku, PM-Scl100, PM-Scl75, Jo-1, SRP, PL-7, PL-12, EJ, OJ, Ro-52.
  • Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster and EUROLineScan.
ProductOrder number
EUROLINE Autoimmune Inflammatory Myopathies 16 Ag DL 1530-1601-4 G

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EUROLINE: Systemic Sclerosis (Nucleoli) Profile

Incubated EUROLINE Systemic Sclerosis Profile (Nucleoli)
  • Differentiation of systemic sclerosis-associated antibodies against cell-nuclear antigens.
  • Indications: systemic sclerosis (SSc, diffuse and limited form), overlap syn­dromes.
  • Serum dilution 1 : 101, conjugate class anti-human IgG, AP-labelled.
  • With the EUROLINE Systemic Sclerosis Profile (Nucleoli), thirteen autoantibodies can be determined: anti­bodies against Scl-70, CENP A, CENP B, RP11 (RNAP-III), RP155 (RNAP-III), fibrillarin, NOR-90, Th/To, PM-Scl100, PM-Scl75, Ku, PDGFR, Ro-52.
  • Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster und EUROLineScan.
ProductOrder number
EUROLINE Systemic Sclerosis (Nucleoli) Profile DL 1532 G

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EUROLINE-WB HEp-2 Cell Antigens plus SS-A, Ro-52 and CENP B

Incubated EUROLINE-WB HEp-2 Cell Antigens plus SS-A, Ro-52 and CENP B
  • Differentiation of antibodies against cell-nuclear and cytoplasmic antigens.
  • Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren’s syndrome, progressive systemic sclerosis, poly/dermatomyositis, PBC.
  • EUROLINE-WB is a combination of westernblot and line blot techniques. Proteins from a whole antigen extract of HEp-2 cells are electrophoretically separated according to molecular mass and transferred onto a nitrocellulose membrane. A membrane chip coated with highly purified native SS-A, recombinant Ro-52 and recombinant CENP B is then added to the westernblot strips.
  • Antibodies against SS-A are characteristic markers for SLE and Sjögren’s syndrome. In contrast, antibodies against Ro-52 also occur in patients with other autoimmune diseases. EUROLINE-WB contains both antigens next to each other at defined positions, in addition to the complete HEp-2 antigen spectrum of the Westernblot. The use of native SS-A increases the sensitivity, since 37% of antibodies against SS-A do not show any reaction with the denatured antigen from the Westernblot 
  • Serum dilution 1 : 51, conjugate class anti-human IgG, AP-labelled.
  • Antigens: SDS extract from HEp-2 cells (whole antigen), highly purified native SS-A from calf thymus, recombinant Ro-52 and CENP B.

ProductOrder number
EUROLINE-WB HEp-2 Cell AntigensDW 1520-3 G

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