Autoantibodies against cell nuclei (ANA)

EUROPLUS ANA Mosaic 20A

EUROPLUS ANA Mosaic 20A (HEp-20-10 cells, primate liver, SS-A+SS-B BIOCHIPs, rib. P-prot.+Jo-1 BIOCHIPs): antibodies against SS-A/SS-B.
  • Screening test for the detection of antibodies against cell nuclei (ANA).
  • Indications: rheumatic diseases.
  • Initial dilution 1 : 100; conjugate class anti-human IgG, FITC-labelled.
  • Using HEp-20-10 cells many antibodies against cell nuclei can be analysed, e.g. antibodies against DNA, histones, RNA, nRNP, Sm, SS-A, SS-B, nuclear dots, centromeres, nuclear membrane, nucleoli (PM-Scl, fibrillarin, RNA polymerase I, NOR), Scl-70, cyclin I and II, spindle fibers, midbody, centrioles.
  • In addition, cytoplasmic autoantibodies are identified with HEp-20-10 cells: antibodies against ribosomes, Jo-1, mitochondria, Golgi apparatus, actin etc.
  • The primate liver permits the verification of results between both substrates, makes the predifferentiation of a large number of ANA possible, and helps to establish titer levels. Moreover, the primate liver contains additional antigens, allowing the identification of further autoantibodies: antibodies against LMA, LSP, endomysium, bile ducts and endothelium cells, as well as cANCA and pANCA.
  • The EUROPLUS system is a monospecific test that can be used to confirm the presence of autoantibodies against cell nuclei and cytoplasm components with one and the same test kit. The following antigens are currently available as EUROPLUS BIOCHIPs: SS-A, SS-B, nRNP/Sm, Sm, Jo-1, ribosomal P-proteins.
ProductOrder number
EUROPLUS ANA Mosaic 20AFA 1512-20

Innovative cell line HEp-20-10 with a high number of mitotic cells

HEp-2010: antibodies against spindle fibers.
  • Screening test for detection of antibodies against cell nuclei.
  • Indications: rheumatic diseases.
  • Initial dilution 1 : 100; conjugate class anti-human IgG, FITC-labelled.
  • Compared to conventional HEp-2 cells, HEp20-10 cells contain ten times as many mitotic cells. Antibodies against mitosis-specific structures (centromeres, spindle fibres, separation zone, centrioles, NOR) can be more easily identified than with conventional preparations.
  • At the same time the cell line HEp-20-10 offers the full antigen spectrum for the detection of cell nuclear antibodies.
  • The HEp-20-10 BIOCHIP can be supplemented with further substrates, for example, primate liver (order number FA 1512-1) or rat kidney and rat stomach (order number FA 1802-3).
  • With EUROPattern, EUROIMMUN offers an innovative and high-performance automation solution for the evaluation of ANA in immunofluorescence.
ProductOrder number
HEp-20-10 BIOCHIPsFA 1522

ANA Screen, Anti-ENA PoolPlus

Incubated ANA Screen ELISA (antigen mixture of dsDNA, histones, nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1, ribosomal P-proteins, centromeres)
  • Screening test for predifferentiation of antibodies against cell nuclei (ANA) and cytoplasm components.
  • Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren's syndrome, progressive systemic sclerosis, polymyositis/dermatomyositis.
  • Serum dilution 1 : 201, conjugate class anti-human IgG, POD-labelled.
  • One microplate well incubated per patient.
  • 1-point calibration, semiquantitative.
  • Native antigens (exception: centromere, recombinant).
  • The ANA Screen ELISA supplements the gold standard immunofluorescence. It is based on a mixture of 10 highly purified antigens, which provide higher sensitivity and specificity than the undefined cell extracts used by other manufacturers.
  • Two ELISAs with different antigen combinations, adapted to particular indications or for follow-up of immunofluorescence patterns, are available.

ProductAntigensOrder number
ANA Screen ELISA (IgG)dsDNA, histones, rib. P-proteins, nRNP/Sm,
Sm, SS-A, SS-B, Scl-70, Jo-1, centromeres
EA 1590-8 G
Anti-ENA PoolPlus ELISA (IgG)   nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1EA 1590-7 G

Anti-ENA SLE Profile 2, Anti-ENA ProfilePlus 1/2

Incubated Anti-ENA ProfilePlus 2 ELISA (antigens: ribosomal P-proteins, nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1, centromeres)
  • Differentiation of antibodies against cell nuclei (ANA) and cytoplasm components.
  • Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren's syndrome, progressive systemic sclerosis, polymyositis/dermatomyositis.
  • Serum dilution 1 : 201; conjugate class anti-human IgG, POD-labelled.
  • 8 or 6 relevant antibodies can be detected simultaneously.
  • 1-point calibration, semiquantitive. Calibrator pool and negative controls each on a separate microplate strip (SLE Profiles and Anti-ENA ProfilePlus 2) or on the same microplate strip as the patient serum.
  • Native antigens (exception: Ro-52, centromere and PM-Scl, recombinant).
  • In total, 4 different ELISAs with different antigen combinations, adapted to particular indications or for follow-up of immunofluorescence patterns, are available.

ProductAntigensOrder number
Anti-ENA ProfilePlus 1 ELISA (IgG)

nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1

EA 1590-1 G
Anti-ENA ProfilePlus 2 ELISA (IgG)rib. P-proteins, nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1, centromeresEA 1590-2 G
Anti-ENA SLE Profile 2 ELISA (IgG)dsDNA, histones, nucleosomes, nRNP/Sm, Sm, SS-A, SS-B, Scl-70EA 1590-12 G

Monospecific ELISA with single antigens

Incubated Anti-SS-A ELISA
  • Differentiation of antibodies against cell nuclei (ANA) and cytoplasm components.
  • Indications: rheumatic diseases.
  • Serum dilution 1 : 201; conjugate class anti-human IgG, POD-labelled.
  • Antibodies against cell nuclei components can be determined quantitatively in RU/ml.
  • 3-point calibration, quantitative.
  • Identical incubation conditions and times: all single-antigen tests can be combined with each other on one microplate.
  • Native antigens (exceptions: centromere and PM-Scl, recombinant).
  • Single-antigen ELISAs available for detection of antibodies against cell nuclei and cytoplasm antigens: ssDNA, nucleosomes, dsDNA, histones, ribosomal P proteins, PM-Scl, nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1, centromere.

EUROLINE: ANA Profile 3

Incubated EUROLINE ANA Profile 3
  • Differentiation of antibodies against cell nuclei (ANA).
  • Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren’s syndrome, systemic sclerosis, poly/dermatomyositis, PBC.
  • Serum dilution 1 : 101; conjugate class anti-human IgG, AP-labelled.
  • With the EUROLINE ANA Profile 3, fifteen autoantibodies can be determined: antibodies against nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70, PM-Scl, Jo-1, centromere protein B, PCNA, dsDNA, nucleosomes, histones, ribosomal P-proteins, AMA M2.
  • Antibodies against SS-A are characteristic markers for SLE and Sjögren’s syndrome. In contrast, antibodies against Ro-52 also occur in patients with other autoimmune diseases.
  • Native antigens, purified by affinity chromatography (exception: centromere protein B, PM-Scl, Ro-52, PCNA).
  • Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster and EUROLineScan.
ProductOrder number
EUROLINE ANA Profile 3 DL 1590-3 G

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EUROLINE: Autoimmune Inflammatory Myopathies 16 Ag

Incubated EUROLINE Autoimmune Inflammatory Myopathies 16 Ag
Incubated EUROLINE Autoimmune Inflammatory Myopathies 16 Ag
  • Differentiation of myositis-associated antibodies against cell-nuclear and cytoplasmic antigens.
  • Indications: poly/dermatomyositis.
  • Serum dilution 1 : 101, conjugate class anti-human IgG, AP-labelled.
  • With the EUROLINE Autoimmune Inflammatory Myopathies 16 Ag, 16 autoantibodies can be determined: antibodies against Mi-2 alpha, Mi-2 beta, TIF1g, MDA5, NXP2, SAE1, Ku, PM-Scl100, PM-Scl75, Jo-1, SRP, PL-7, PL-12, EJ, OJ, Ro-52.
  • Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster and EUROLineScan.
ProductOrder number
EUROLINE Autoimmune Inflammatory Myopathies 16 Ag DL 1530-1601-4 G

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EUROLINE: Systemic Sclerosis (Nucleoli) Profile

Incubated EUROLINE Systemic Sclerosis Profile (Nucleoli)
  • Differentiation of systemic sclerosis-associated antibodies against cell-nuclear antigens.
  • Indications: systemic sclerosis (SSc, diffuse and limited form), overlap syn­dromes.
  • Serum dilution 1 : 101, conjugate class anti-human IgG, AP-labelled.
  • With the EUROLINE Systemic Sclerosis Profile (Nucleoli), thirteen autoantibodies can be determined: anti­bodies against Scl-70, CENP A, CENP B, RP11 (RNAP-III), RP155 (RNAP-III), fibrillarin, NOR-90, Th/To, PM-Scl100, PM-Scl75, Ku, PDGFR, Ro-52.
  • Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster und EUROLineScan.
ProductOrder number
EUROLINE Systemic Sclerosis (Nucleoli) Profile DL 1532 G

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EUROLINE-WB HEp-2 Cell Antigens plus SS-A, Ro-52 and CENP B

Incubated EUROLINE-WB HEp-2 Cell Antigens plus SS-A, Ro-52 and CENP B
  • Differentiation of antibodies against cell-nuclear and cytoplasmic antigens.
  • Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren’s syndrome, progressive systemic sclerosis, poly/dermatomyositis, PBC.
  • EUROLINE-WB is a combination of westernblot and line blot techniques. Proteins from a whole antigen extract of HEp-2 cells are electrophoretically separated according to molecular mass and transferred onto a nitrocellulose membrane. A membrane chip coated with highly purified native SS-A, recombinant Ro-52 and recombinant CENP B is then added to the westernblot strips.
  • Antibodies against SS-A are characteristic markers for SLE and Sjögren’s syndrome. In contrast, antibodies against Ro-52 also occur in patients with other autoimmune diseases. EUROLINE-WB contains both antigens next to each other at defined positions, in addition to the complete HEp-2 antigen spectrum of the Westernblot. The use of native SS-A increases the sensitivity, since 37% of antibodies against SS-A do not show any reaction with the denatured antigen from the Westernblot 
  • Serum dilution 1 : 51, conjugate class anti-human IgG, AP-labelled.
  • Antigens: SDS extract from HEp-2 cells (whole antigen), highly purified native SS-A from calf thymus, recombinant Ro-52 and CENP B.

ProductOrder number
EUROLINE-WB HEp-2 Cell AntigensDW 1520-3 G

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EUROASSAY: Anti-ENA ProfilePlus

Incubated EUROASSAY Anti-ENA ProfilePlus
  • Differentiation of anti-nuclear antibodies (ANA).
  • Indications: rheumatic diseases.
  • Serum dilution 1 : 101; conjugate class anti-human IgG, AP-labelled.
  • 6 relevant anti-nuclear antibodies against "extractable nuclear antigens" (ENA) can be detected simultaneously and monospecifically: antibodies against nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1.
  • Native antigens, purified by affinity chromatography.
  • On request EUROASSAY can be produced with special antigen combinations, for example, with dsDNA, histones, nucleosomes, PM-Scl, nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70, Jo-1, ribosomal P proteins, centromere protein B, M2, M4, M9, SLA/LP, LC-1, LKM-1.

ProductOrder number
EUROASSAY Anti-ENA ProfilePlus  DA 1590-1 G