Indirect immunofluorescence: an easy and modern method

Immunofluorescence tests from EUROIMMUN: high-tech, not old-fashioned! Numerous innovations contribute to
the standardisation and modernisation of indirect immunofluorescence:

Activation technique:
Physically or chemically activated cover glasses are coated with cultured cells or tissue sections. Frozen tissue sections are fixed to the glass surface by covalent bonding. This increases the adhesion by more than 100 fold, preventing detachment of the sections.

BIOCHIP Technology:
Cover glasses coated with biological substrates are cut mechanically into millimetre-sized fragments (BIOCHIPs). Ten or more first-class preparations of consistent quality can be obtained per tissue section, for cultured cell substrates even several thousands.

BIOCHIP Mosaics:
When multiple BIOCHIPs coated with different substrates are arranged in one reaction field, antibodies against various organs or infectious agents can be investigated simultaneously. Comprehensive antibody profiles can be easily established (multiplex) and the results are verified reciprocally on different substrates.

The samples or reagents are first pipetted onto the reaction fields of a reagent tray. The slides are then placed into recesses of the reagent tray, where all BIOCHIPs come into contact with the liquids, and the individual reactions begin simultaneously. As the fluids are confined in a closed space, there is no need for a conventional humidity chamber.

EUROIMMUN offers a range of IFT automation options for both low and high throughput, from sample dilution to fully automated evaluation of fluorescence images, including archiving.