ChLIA
Euroimmun ChLIA
Random access and highest speed
Advantages of Euroimmun ChLIA
- Fully automated processing from primary sample to result using the IDS-iSYS Multi-Discipline Automated System or the IDS-i10
- Highest speed – results in just 25 minutes
- Ready-for-use reagent cartridges and calibrators with long stability, also cooled in the instrument
- Transmission of quality control data for error-free and quick loading with little manual effort
- Quantification based on calibration curves which are stable over several weeks
- Broad dynamic analytical range due to high signal intensity at high sensitivity and specificity
Test principle
Chemiluminescence immunoassays (ChLIA) from Euroimmun use magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals. Performance and evaluation are fully automated with the IDS-iSYS or the IDS-i10.
Antibody detection by ChLIA
The antigen-coated magnetic particles are incubated with diluted patient samples. If a sample contains specific antibodies directed against the antigen, these bind to the antigen-coated magnetic particles. In the next step, acridinium-labelled antibody (conjugate) is added. This binds to the specific antibodies. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antibody concentration in the patient sample within the measurement range and is automatically converted into a concentration by the instrument.
Antigen detection by ChLIA
The antibody-coated magnetic particles are incubated with the patient sample and an antigen-specific biotinylated antibody. During the incubation, the antigen is bound by the magnetic particle-coupled antibody as well as by the biotinylated antibody. In the next step, acridinium-labelled ExtrAvidin (conjugate) is added. This binds to the biotinylated antibody. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antigen concentration in the patient sample within the indicated measurement range and is converted automatically into a concentration by the instrument.
