Chronic inflammatory bowel diseases

Clinical information

The most important chronic inflammatory bowel diseases (CIBD) include ulcerative colitis (UC) and Crohn’s disease (CD).

Ulcerative colitis (UC) belongs to the CIBD with autoimmune reactions against the mucosa and submucosa of the colon or rectum and increased immune reactions against the intestinal flora. It is assumed that there is a genetic susceptibility to UC and that it may also be caused by certain environmental factors. The inflammation spreads continuously from the rectum, i.e. from anal to oral.

CD is classified as an autoimmune disease of the intestinal mucosa and is among the CIBD with a high recurrence rate. The chronic granulomatous inflammation, which can affect the whole digestive tract from the oral cavity to the anus, is found in most cases only in the lower small intestine (terminal ileum) and the large intestine (colon), very rarely in the oesophagus and mouth. A discontinuous, segmental attack on the intestinal mucosa, which may affect several separate sections of the intestine simultaneously, is characteristic for CD.

Diagnostics

Initial differentiation between irritable bowel syndrome (IBS) and CIBD is possible by non-invasive antigen detection of calprotectin in stool, which thus allows a crucial precharacterisation of intestine-associated inflammations (see chapter “Calprotectin”). The high diagnostic requirements of a differentiated CIBD determination are met by IIF single assays as well as by various highly specific mosaics (CIBD profiles), which have been developed especially for serological diagnostics of CD and UC.

Autoantibodies against the exocrine pancreas are a reliable marker for CD. They have a high disease-specific significance due to their organ specificity, disease association and frequently high serum concentration. Since the inflammation of the intestinal wall in CD is caused by the autoantigens contained in the pancreas secretion, particularly the proteoglycans CUZD1 and GP2, the determination of autoantibodies against the pancreas antigens rPAg1 (CUZD1) and/or PAg2 (GP2) using IFA represents a new dimension in serological CD diagnostics. Anti-Saccharomyces cerevisiae antibodies (ASCA) are another specific parameter that supports serological CD diagnostics.

Autoantibodies against intestinal goblet cells, which occur exclusively in UC, are pathognomonic markers for this autoimmune disease. The target antigen responsible for UC has not yet been exactly identified. The serological determination of autoantibodies against DNA-bound lactoferrin contributes significantly to the diagnosis of CIBD, particularly of UC.

Methods

ChLIA (5)

ELISA (3)

EUROLINE (2)

IIFT (7)

NUL (3)

Technology	Parameter	Substrate	Species	Calibration	Ig Class	Format	Order No.
EUROLINE	EUROLINE Autoimmune Gastrointestinal Diseases (IgA) (tissue transglutaminase (endomysium), gliadin-analogue fusion peptide (GAF-3X), mannan (ASCA))	EUROLINE			IgA IgA IgA	05 x 10 16 x 01 64 x 01	DL 1360-0510 A DL 1360-1601 A DL 1360-6401 A
EUROLINE	EUROLINE Autoimmune Gastrointestinal Diseases (IgG) (tissue transglutaminase (endomysium), gliadin-analogue fusion peptide (GAF-3X), parietal cell antigen (PCA) separately Intrinsic factor, mannan (ASCA))	EUROLINE			IgG IgG IgG	16 x 01 50 x 01 64 x 01	DL 1360-1601 G DL 1360-5001 G DL 1360-6401 G
IIFT	antibodies against pancreas acini (CUZD1 control; associated with Crohn's disease)				IgG	05 x 02	CA 1391-0502
IIFT	CIBD Screen 3 pancreas ag rPAg1(CUZD1) / rPAg2(GP2) intestinal goblet cells	3 BIOCHIPs per field: transfected cells goblet cells control transfection	EU 90 EU 80 EU 90		IgA/G	10 x 05 10 x 05	FA 1391-1005-3 FB 1391-1005-3
IIFT	CIBD Profile 7 upper row: pancreas ag rPAg1(CUZD1) / rPAg2(GP2) intestinal goblet cells pANCA bottom row: Saccharomyces cerevisiae	transfected cells goblet cells granulocytes (EOH) control transfection fungal smear	EU 90 EU 80 human EU 90 S. cerevisiae		IgA/G	10 x 05 10 x 05	FA 1391-1005-7 FB 1391-1005-7
ChLIA	IDS Calprotectin Sample type: Stool	Antibody-coated magnetic particles	Calprotectin	quantitative		100 x 01	IS-AI26000¹
ChLIA	IDS Calprotectin Calibrator Set	2 x 0.5 ml calibrator 1/2/3/4/5	Calprotectin			5 concentration levels	IS-AI26020¹
ChLIA	IDS Calprotectin Control Set	3 x 0.5 ml control 1/2	Calprotectin			2 concentration levels	IS-AI26030¹
ELISA	calprotectin determination in stool	Antibody-coated microplate wells		0/15/60/240/960/2100 µg/g		96 x 01	EQ 6831-9601 W
ChLIA	Faecal Calprotectin ChLIA Sample type: Stool	Antibody-coated magnetic particles		quantitative		01 x 00	LQ 6831-0100 W
ChLIA	Control set Faecal Calprotectin ChLIA	2 x 0.5 ml control 1/2				02 x 02	LR 6831-0160 W
NUL	IDS Calprotectin Extraction Device Use in combination with IS-AI26000	Use in combination with IS-AI26000				01 x 01	IS-AI26040¹
NUL	Stool Dosage Tubes, unfilled Use in combination with EQ / LQ 6831 W	Use in combination with EQ / LQ 6831 W				100 x 01	ZE 6010-0100-3
NUL	Stool Dosage Tubes, ready for use Use in combination with EQ / LQ 6831 W	Use in combination with EQ / LQ 6831 W				45 x 01	ZE 6010-4501-2
ELISA	Anti-Saccharomyces cerevisiae ELISA (IgA)	antigen-coated microplate wells	mannan of S. cerevisiae	2/20/200 RU/ml	IgA	96 x 01	EV 2841-9601 A
ELISA	Anti-Saccharomyces cerevisiae ELISA (IgG)	antigen-coated microplate wells	mannan of S. cerevisiae	2/20/200 RU/ml	IgG	96 x 01	EV 2841-9601 G
IIFT	antibodies against Saccharomyces cerevisiae IgA positive control	fungal smear	Saccharomyces cerevisiae		IgA	05 x 02	CV 2841-0502 A
IIFT	antibodies against Saccharomyces cerevisiae IgG positive control	fungal smear	Saccharomyces cerevisiae		IgG	05 x 02	CV 2841-0502 G
IIFT	Saccharomyces cerevisiae negative control				IgA, IgG, IgM	05 x 02	CV 2841-0502 Z
IIFT	Saccharomyces cerevisiae	fungal smear	Saccharomyces cerevisiae		IgA IgG IgA IgG IgA IgG	10 x 05 10 x 10 20 x 05 10 x 05 10 x 05 10 x 10 10 x 10 20 x 05 20 x 05	FX 2841-1005 FX 2841-1010 FX 2841-2005 FV 2841-1005 A FV 2841-1005 G FV 2841-1010 A FV 2841-1010 G FV 2841-2005 A FV 2841-2005 G
* For research use only, not for in vitro diagnostic use in the sense of EU directive 98/79/EC. ¹ Product manufactured by third party. Please contact your local Euroimmun representative for more information.