Herpes virus infections

Clinical information

The family of herpesviruses encompasses more than 100 different virus types, eight of which can cause infections in humans. These so-called human herpesviruses encompass herpes simplex virus of type 1 and 2 (HSV-1 and -2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesviruses 6 and 7 (HHV-6 and -7) as well as human herpesvirus 8 (HHV-8), which is associated with Kaposi sarcoma. Despite great structural similarities of the different pathogens, herpesvirus infection can cause very different diseases, e.g. cold sores or genital herpes, chickenpox, shingles, kissing disease or different cancers. During pregnancy, especially primary infection with particular herpesviruses may lead to severe damage or even loss of the child. There is currently only a vaccine against VZV. After primary infection, which often proceeds asymptomatically, herpesviruses persist life-long in the body in a latent state and may become reactivated at a later time point, e.g. due to a weakened immune system, and (again) cause symptoms.

Diagnostics

Laboratory diagnostics of herpesvirus infections is based on direct or indirect detection methods. Direct methods, which are especially relevant for diagnostics of acute infections or in immunodeficiency include, e.g. virus culture or PCR-based tests, in which viral nucleic acids are detected. Blister fluid, skin or mucosal smears, blood, CSF or broncoalveolar lavage fluid is used as sample material. If the herpesvirus specific antibody is detected in serum, plasma or CSF, this is considered as indirect pathogen detection. The antibody detection not only allows conclusions to be drawn on which virus is present in the body of the patient but can also contribute to the differentiation of primary and recurrent infections. EUROIMMUN offers a broad spectrum of products for herpesvirus infection diagnostics including both antibody detection and direct pathogen detection.

Methods

ChLIA (29)

ELISA (30)

EUROLINE (10)

IIFT (8)

MICROBLOT (2)

WESTERNBLOT (3)

Technology	Parameter	Substrate	Species	Calibration	Ig Class	Format	Order No.
ELISA	CSQ pair of controls anti-Cytomegalovirus (IgG)				IgG	02 x 20	CK 2570-0220-L G
ELISA	CSQ pair of controls anti-EBV-CA (IgG)				IgG	02 x 20	CK 2791-0220-L G
ELISA	CSF: Anti-CMV ELISA (IgG) cytomegalovirus Sample type: CSF/serum pair	antigen-coated microplate wells	native antigens from CMV	5/25/50/100 U	IgG	96 x 01	EI 2570-9601-L G
ELISA	CSF: Anti-EBV-CA-ELISA (IgG) Epstein-Barr virus capsid antigen Sample type: CSF/serum pair	antigen-coated microplate wells	native, mixture of several viral capsid antigens	5/25/50/100 U	IgG	96 x 01	EI 2791-9601-L G
ELISA	CSQ pair of controls anti-HSV-1/2 Pool (IgG)				IgG	02 x 20	CK 2531-0220-1 L G
ELISA	CSF: Anti-HSV-1/2 Pool ELISA (IgG) herpes simplex virus Sample type: CSF/serum pair	antigen-coated microplate wells	native antigens from HSV-1 and HSV-2	5/25/50/100/175/230 U/ml	IgG	96 x 01	EI 2531-9601-1 L G
ELISA	CSQ pair of controls anti-VZV (IgG)				IgG	02 x 20	CK 2650-0220-L G
IIFT	HHV-6	infected cells	EU 30		IgG IgM IgG IgM IgG IgM IgG IgM	10 x 05 10 x 05 10 x 10 10 x 10 20 x 05 20 x 05 20 x 10 20 x 10	FI 2536-1005 G FI 2536-1005 M FI 2536-1010 G FI 2536-1010 M FI 2536-2005 G FI 2536-2005 M FI 2536-2010 G FI 2536-2010 M
IIFT	Epstein-Barr virus capsid antigen (EBV-CA)	expressing cells	P3HR1		IgG EB IgM EB IgG EB IgM EB IgM EB IgG EB	10 x 05 10 x 05 10 x 10 10 x 10 20 x 05 20 x 10	FI 2791-1005 G FI 2791-1005 M FI 2791-1010 G FI 2791-1010 M FI 2791-2005 M FI 2791-2010 G
IIFT	Epstein-Barr virus nuclear antigen (EBNA, complement-fixing antibodies)	expressing cells	Raji		C3c EB	10 x 10	FI 2793-1010 C
IIFT	Epstein-Barr virus early antigen (EBV-EA)	expressing cells	EU 33		IgG EB IgG EB	10 x 05 10 x 10	FI 2795-1005 G FI 2795-1010 G
IIFT	BIOCHIP Sequence EBV fields A and B: EBV-CA (IgG) field C: EBV-CA (IgM) field D: EBV-EA field E: EBNA	expressing cells expressing cells expressing cells expressing cells	P3HR1 P3HR1 EU 33 Raji		Aviditätsbestimmung Aviditätsbestimmung Aviditätsbestimmung Aviditätsbestimmung	10 x 01 10 x 02 20 x 01 20 x 02	FI 2799-1001-1 X FI 2799-1002-1 X FI 2799-2001-1 X FI 2799-2002-1 X
IIFT	EUROPLUS BIOCHIP Sequence EBV field A: EBV-CA (IgG), gp125 ag, p19 ag field B: EBV-CA (IgG) field C: EBV-CA (IgM), gp125 ag, p19 ag field D: EBV-EA field E: EBNA	expr. cells, gp125/p19/ ag-free BIOCHIP expressing cells expressing cells expr. cells, gp125/p19/ ag-free BIOCHIP expressing cells	P3HR1, native/rec. EU 120 P3HR1 P3HR1, native/rec. EU 120 EU 33 Raji		Aviditätsbestimmung Aviditätsbestimmung Aviditätsbestimmung Aviditätsbestimmung	10 x 01 10 x 02 20 x 01 20 x 02	FI 2799-1001-21 X FI 2799-1002-21 X FI 2799-2001-21 X FI 2799-2002-21 X
IIFT	HHV-6 EUROPattern	infected cells	EU 30		IgG EB IgM EB IgG EB IgM EB IgG EB IgM EB IgG EB IgM EB	10 x 05 10 x 05 10 x 10 10 x 10 20 x 05 20 x 05 20 x 10 20 x 10	FR 2536-1005 G FR 2536-1005 M FR 2536-1010 G FR 2536-1010 M FR 2536-2005 G FR 2536-2005 M FR 2536-2010 G FR 2536-2010 M
ELISA	Anti-EBV-CA ELISA (IgA) Epstein-Barr virus capsid antigen	antigen-coated microplate wells	native, mixture of several viral capsid antigens	semi-quantitative	IgA	96 x 01	EI 2791-9601 A
ELISA	Anti-EBV-CA ELISA (IgG) Epstein-Barr virus capsid antigen	antigen-coated microplate wells	native, mixture of several viral capsid antigens	2/20/200 RU/ml	IgG	96 x 01	EI 2791-9601 G
ELISA	Anti-EBV-CA ELISA (IgM) Epstein-Barr virus capsid antigen	antigen-coated microplate wells	native gp125 of EBV	semi-quantitative	IgM	96 x 01	EI 2791-9601 M
ELISA	Avidity: Anti-EBV-CA-ELISA (IgG) Epstein-Barr virus capsid antigen avidity determination	antigen-coated microplate wells	native, mixture of several viral capsid antigens	2/20/200 RU/ml	IgG	96 x 01	EI 2791-9601-1 G
ELISA	Anti-EBNA-1 ELISA (IgG) Epstein-Barr virus nuclear antigen	antigen-coated microplate wells	recombinant EBNA-1 from EBV	2/20/200 RU/ml	IgG	96 x 01	EI 2793-9601 G
ELISA	Anti-EBV-EA-D ELISA (IgA) Epstein-Barr virus early antigen	antigen-coated microplate wells	recombinant early antigen D from EBV	semi-quantitative	IgA	96 x 01	EI 2795-9601 A
ELISA	Anti-EBV-EA-D ELISA (IgG) Epstein-Barr virus early antigen	antigen-coated microplate wells	recombinant early antigen D from EBV	2/20/200 RU/ml	IgG	96 x 01	EI 2795-9601 G
ELISA	Anti-EBV-EA-D ELISA (IgM) Epstein-Barr virus early antigen	antigen-coated microplate wells	recombinant early antigen D from EBV	semi-quantitative	IgM	96 x 01	EI 2795-9601 M
EUROLINE	positive control serum: IgG, human, 50x concentrated for EBV-Profil 2 G				IgG	05 x 07	CL 2790-0507-12 G
EUROLINE	positive control serum: IgM, human, 50x concentrated for EBV-Profil 2 M				IgM	05 x 07	CL 2790-0507-12 M
EUROLINE	EUROLINE Anti-EBV Profile 2 (IgM) (VCA gp125, VCA p19, EBNA-1, p22, EA-D separately)	EUROLINE			IgM IgM IgM	05 x 10 16 x 01 64 x 01	DN 2790-0510-2 M DN 2790-1601-2 M DN 2790-6401-2 M
EUROLINE	EUROLINE Anti-EBV (IgM) (VCA gp125, VCA p19, EA-D separately)	EUROLINE			IgM IgM IgM	05 x 10 16 x 01 64 x 01	DN 2790-0510-4 M DN 2790-1601-4 M DN 2790-6401-4 M
EUROLINE	EUROLINE Anti-EBV Profile 2 (IgG) (VCA gp125, VCA p19, EBNA-1, p22, EA-D separately)	EUROLINE			IgG IgG IgG	16 x 01 50 x 01 64 x 01	DN 2790-1601-2 G DN 2790-5001-2 G DN 2790-6401-2 G
EUROLINE	EUROLINE Anti-EBV (IgG) (VCA gp125, VCA p19, EBNA-1, p22. EA-D separately)	EUROLINE			IgG IgG IgG	16 x 01 50 x 01 64 x 01	DN 2790-1601-4 G DN 2790-5001-4 G DN 2790-6401-4 G
ChLIA	IDS EBV VCA IgG	antigenic coated magnetic particles	Capsid antigen	quantitative		100 x 01	IS-ID5801¹
ChLIA	IDS EBV VCA IgM	antigenic coated magnetic particles	Capsid antigen	quantitative		100 x 01	IS-ID5802¹
ChLIA	IDS EBV EBNA-1 IgG	antigenic coated magnetic particles	EBNA-1	quantitative		100 x 01	IS-ID5803¹
ChLIA	IDS EBV EA IgG	antigenic coated magnetic particles	EA-D antigen	quantitative		50 x 01	IS-ID5804¹
ChLIA	IDS EBV Control Set	3 x 1.5 ml Control 1/2	human serum			2 concentrations	IS-ID5830¹
ChLIA	Anti-EBV-CA ChLIA (IgG) Epstein-Barr virus capsid antigen	Antigen-coated magnetic particles	recombinant p18, p23, gp125	quantitative	IgG	100 x 1	LI 2791-10010 G
ChLIA	Anti-EBV-CA ChLIA (IgM) Epstein-Barr virus capsid antigen	Antigen-coated magnetic particles	recombinant p18, gp125	quantitative	IgM	100 x 1	LI 2791-10010 M
ChLIA	Anti-EBNA-1 ChLIA (IgG) Epstein-Barr virus nuclear antigen	Antigen-coated magnetic particles	recombinant EBNA-1	quantitative	IgG	100 x 1	LI 2793-10010 G
ChLIA	Control set Anti-EBV-CA ChLIA (IgG) Epstein-Barr virus capsid antigen	2 x 0.5 ml control 1/2			IgG	02 x 02	LR 2791-20210 G
ChLIA	Control set Anti-EBV-CA ChLIA (IgM) Epstein-Barr virus capsid antigen	2 x 0.5 ml control 1/2			IgM	02 x 02	LR 2791-20210 M
ChLIA	Control set Anti-EBNA-1 ChLIA (IgG) Epstein-Barr virus nuclear antigen	2 x 0.5 ml control 1/2			IgG	02 x 02	LR 2793-20210 G
MICROBLOT	EUROMicroblot Anti-EBV (IgG)				IgG	96 x 01	KN 2790-9601 G
MICROBLOT	EUROMicroblot Anti-EBV (IgM)				IgM	96 x 01	KN 2790-9601 M
ELISA	Anti-CMV ELISA (IgG) cytomegalovirus	antigen-coated microplate wells	native antigens from CMV	2/20/200 RU/ml	IgG	96 x 01	EI 2570-9601 G
ELISA	Anti-CMV ELISA (IgM) cytomegalovirus	antigen-coated microplate wells	native antigens from CMV	semi-quantitative	IgM	96 x 01	EI 2570-9601 M
ELISA	Avidity: Anti-CMV ELISA (IgG) cytomegalovirus avidity determination	antigen-coated microplate wells	native antigens from CMV	2/20/200 RU/ml	IgG	96 x 01	EI 2570-9601-1 G
ELISA	Anti-CMV p52 ELISA (IgM) cytomegalovirus p52 antigen from CMV	antigen-coated microplate wells	native antigens from CMV	semi-quantitative	IgM	96 x 01	EI 2570-9601-2 M
ELISA	Anti-CMV gB ELISA (IgG) cytomegalovirus glycoprotein B glycoprotein B antigen from CMV	antigen-coated microplate wells	recombinant glycoprotein B antigen from CMV	2/20/200 RU/ml	IgG	96 x 01	EI 2570-9601-3 G
EUROLINE	positive control serum: IgG, human, 50x concentrated for CMV				IgG	05 x 07	CL 2570-0507 G
EUROLINE	positive control serum: IgM, human, 50x concentrated for CMV				IgM	05 x 07	CL 2570-0507 M
EUROLINE	EUROLINE Anti-CMV (IgG)	EUROLINE			IgG IgG IgG	16 x 01 50 x 01 64 x 01	DN 2570-1601-2 G DN 2570-5001-2 G DN 2570-6401-2 G
EUROLINE	EUROLINE Anti-CMV (IgM)	EUROLINE			IgM IgM IgM	16 x 01 50 x 01 64 x 01	DN 2570-1601-2 M DN 2570-5001-2 M DN 2570-6401-2 M
ChLIA	IDS CMV IgG	antigenic coated magnetic particles	CMV antigen	qualitative		100 x 01	IS-ID5401¹
ChLIA	IDS CMV IgM	antigenic coated magnetic particles	CMV antigen	qualitative		100 x 01	IS-ID5402¹
ChLIA	IDS CMV Control Set	3 x 1.5 ml Control 1/2	human serum			2 concentrations	IS-ID5430¹
ChLIA	IDS CMV IgG Avidity	antigenic coated magnetic particles	CMV antigen	qualitative		50 x 01	IS-ID5501¹
ChLIA	IDS CMV Avidity Control Set	3 x 1.0 ml Control 1/2	human serum			2 concentrations	IS-ID5530¹
ELISA	Anti-HSV-1/2 Pool ELISA (IgG) herpes simplex virus native antigens from HSV-1 and HSV-2	antigen-coated microplate wells	native antigens from HSV-1 and HSV-2	2/20/200 RU/ml	IgG	96 x 01	EI 2531-9601-1 G
ELISA	Anti-HSV-1/2 Pool ELISA (IgM) herpes simplex virus native antigens from HSV-1 and HSV-2	antigen-coated microplate wells	native antigens from HSV-1 and HSV-2	semi-quantitative	IgM	96 x 01	EI 2531-9601-1 M
ELISA	Anti-HSV-1 (gC1) ELISA (IgG) herpes simplex virus 1 recombinant glycoprotein C1 from HSV-1	antigen-coated microplate wells	recombinant glycoprotein C1	2/20/200 RU/ml	IgG	96 x 01	EI 2531-9601-2 G
ELISA	Anti-HSV-2 (gG2) ELISA (IgG) herpes simplex virus 2 recombinant glycoprotein G2 from HSV-2	antigen-coated microplate wells	recombinant glycoprotein G2	2/20/200 RU/ml	IgG	96 x 01	EI 2532-9601-2 G
WESTERNBLOT	antibodies against Herpes simplex virus (HSV) IgG positive control				IgG	50 x 05	CW 2531-5005 G
WESTERNBLOT	Anti-HSV-1 / HSV-2 gG-2 EUROLINE-WB (IgG) Herpes simplex virus 1 (HSV-1) plus HSV-2 type-specific glycoprotein G2	Westernblot	whole antigen, SDS extract of HSV-1 plus purified gG2		IgG	16 x 01	DY 2531-1601-1 G
WESTERNBLOT	Anti-HSV-1 / HSV-2 gG-2 EUROLINE-WB (IgM) Herpes simplex virus 1 (HSV-1) plus HSV-2 type-specific glycoprotein G2	Westernblot	whole antigen, SDS extract of HSV-1 plus purified gG2		IgM	16 x 01	DY 2531-1601-1 M
ChLIA	IDS HSV-1 IgG	antigenic coated magnetic particles	gG1 antigen	quantitative		100 x 01	IS-ID5601¹
ChLIA	IDS HSV-2 IgG	antigenic coated magnetic particles	gG2 antigen	quantitative		100 x 01	IS-ID5602¹
ChLIA	IDS HSV 1/2 IgG Control Set	3 x 1.5 ml Control 1/2	human serum			2 concentrations	IS-ID5630¹
ChLIA	IDS HSV 1/2 IgM	antigenic coated magnetic particles	gG1 and gG2 antigens	quantitative		100 x 01	IS-ID5701¹
ChLIA	IDS HSV 1/2 IgM Control Set	3 x 1.5 ml Control 1/2	human serum			2 concentrations	IS-ID5730¹
ChLIA	Anti-Herpes simplex virus 1 ChLIA (IgG)	Antigen-coated magnetic particles	recombinant gG1	quantitative	IgG	100 x 1	LI 2531-10010 G
ChLIA	Anti-Herpes simplex virus 2 ChLIA (IgG)	Antigen-coated magnetic particles	rekombinant gG2	quantitative	IgG	100 x 1	LI 2532-10010 G
ChLIA	Control set Anti-HSV 1 ChLIA (IgG)	2 x 0.5 ml control 1/2			IgG	02 x 02	LR 2531-20210 G
ChLIA	Control set Anti-HSV 2 ChLIA (IgG)	2 x 0.5 ml control 1/2			IgG	02 x 02	LR 2532-20210 G
IIFT	Varicella zoster virus (VZV)	infected cells	EU 168		IgG IgM IgG IgM	10 x 05 10 x 05 10 x 10 10 x 10	FI 2650-1005 G FI 2650-1005 M FI 2650-1010 G FI 2650-1010 M
ELISA	Anti-VZV ELISA (IgA) Varicella zoster virus	antigen-coated microplate wells	native antigens from VZV	semi-quantitative	IgA	96 x 01	EI 2650-9601 A
ELISA	Anti-VZV ELISA (IgG) Varicella zoster virus	antigen-coated microplate wells	native antigens from VZV	10/100/500/5000 IU/l	IgG	96 x 01	EI 2650-9601 G
ELISA	Anti-VZV ELISA (IgM) Varicella zoster virus	antigen-coated microplate wells	native antigens from VZV	semi-quantitative	IgM	96 x 01	EI 2650-9601 M
ELISA	Avidity: Anti-VZV ELISA (IgG) Varicella zoster virus avidity determination	antigen-coated microplate wells	native antigens from VZV	10/100/500/5000 IU/l	IgG	96 x 01	EI 2650-9601-1 G
ELISA	Anti-VZV ELISA 2.0 (IgG) Varicella zoster virus	antigen-coated microplate wells	native antigens from VZV		IgG	96 x 01	EI 2650-9601-2 G
ELISA	Anti-VZV Glycoprotein ELISA (IgM) Varicella zoster virus	antigen-coated microplate wells	native glycoproteins from VZV	semi-quantitative	IgM	96 x 01	EI 2650-9601-2 M
ChLIA	IDS VZV IgG	antigenic coated magnetic particles	VZV virus antigen	quantitative		50 x 01	IS-ID5903¹
ChLIA	IDS MMV IgG Control Set	3 x 1.5 ml Control 1/2	human serum			2 concentrations	IS-ID5930¹
ChLIA	IDS VZV IgM	antigenic coated magnetic particles	VZV virus antigen	quantitative		50 x 01	IS-ID6003¹
ChLIA	IDS MMV IgM Control Set	3 x 1.5 ml Control 1/2	human serum			2 concentrations	IS-ID6030¹
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