Autoantibodies against desmoglein 1, desmoglein 3, envo­plakin, BP180, BP230, collagen type VII

Dermatology Mosaic 7

Dermatology Mosaic 7: oesophagus and split skin (top and middle left), BP230 gC and BP180-NC16A-4X (top and middle right), desmoglein 1 and 3 (bottom left and right): antibodies against epidermal basement membrane
  • Screening and differentiation test for detection of skin-specific antibodies.
  • Indication: bullous autoimmune dermatoses.
  • Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
  • The BIOCHIP Mosaic consists of 6 substrates: primate oesophagus, salt-split skin, desmoglein-1-expressing cells, desmoglein-3-expressing cells, BP230-expressing cells (gc) and BP180-NC16A-4X (EUROPLUS). Thus a comprehensive antigen spectrum is available in a single analysis, allowing targeted serological diagnosis.
  • Antibodies against prickle cell desmosomes react with surface antigens of keratinocytes. Tissue sections of oesophagus and tongue show a granular fluo­rescence of the intercellular matter in the whole stratum spinosum, but differentiation between desmoglein 1 and desmoglein 3 is difficult. When specific transfected cells are employed in addition, a targeted diagnosis in a single test run is possible.
  • Antibodies against basal membrane structures react with salt-split skin. Anti-BP180, anti-BP230 and anti-LAD97 cause staining of the epidermal side, and antibodies against laminin 5, collagen type VII and other antigens staining of the dermal side of the salt-split skin.
  • When autoantibodies against BP180 or BP230 are present, the epidermal basement membrane in the oesophagus or tongue is visible as a fine linear colouring between the stratum basale and the connective tissue. These antibodies can be differentiated by means of BP180-NC16A-4X coated BIOCHIPs and cells specifically transfected with BP230 (globular C-terminal domain (gC)).
  • This BIOCHIP Mosaic can be customised with further substrates if required, e.g. tongue (antibodies against prickle cell desmosomes, epidermal basement membrane), bladder (antibodies against plakins), collagen type VII NCI-expressing cells.

ProductOrder number
Dermatology Mosaic 7FA 1501-7
Dermatology Mosaics EUROPattern FC 1501

Anti-desmoglein 1, -desmoglein 3, -envoplakin, -BP180-NC16A-4X, -BP230-CF and -collagen type VII

  • Monospecific detection of antibodies against desmoglein 1, desmoglein 3, envo­plakin, BP180, BP230 or collagen type VII.
  • Indication: bullous autoimmune dermatoses
  • Serum dilution 1:101; conjugate class anti-human IgG, POD-labelled.
  • 3-point calibration, quantitative (exception: anti-envoplakin and anti-collagen type VII, Dermatology Profile ELISA, semiquantitative).
  • Identical incubation conditions and times: all tests can be combined on one microplate.
  • Recombinant antigens: extracellular domain of desmoglein 1 or 3, envoplakin, tetramer of NC16A domain of BP180 protein, C-terminal segment of BP230 protein, anti-collagen type VII. The corresponding human cDNA is produced in E. coli (BP180-NC16A-4X, BP230-CF, envoplakin and anti-collagen type VII) or in mammalian cells (desmoglein 1, desmoglein 3).
  • Multiplex analysis using the Dermatology-Profile-ELISA (IgG) for the determination of 6 different autoantibodies in only one run: desmoglein 1, desmoglein 3, envo¬plakin, BP180, BP230 and collagen type VII.

(Kopie 1)

ProductOrder number
Dermatology Profile ELISAEA 1490-1 G
Anti-Desmoglein 1 ELISAEA 1495 G
Anti-Desmoglein 3 ELISAEA 1496 G
Anti-Envoplakin ELISAEA 1491 G
Anti-BP230-CF ELISAEA 1502-1 G
Anti-BP180-NC16A-4X ELISAEA 1502 G
Anti-Collagen Type VII ELISAEA 1947 G