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Connective tissue diseases (CTD) are rheumatic diseases. They include, for example, systemic lupus erythematosus, systemic sclerosis, Sjögren's syndrome, myositis syndromes or Sharp syndrome.
Autoantibodies against cellular components, commonly called anti-nuclear antibodies (ANA), are important serological markers for CTD. Target antigens include nucleic acids, cell nuclear proteins and ribonuclear proteins as well as components of the cytoplasm. The frequency (prevalence) of ANA in inflammatory rheumatic diseases lies between 20% and 100%. Differential ANA diagnostics is therefore indispensable for the identification of individual rheumatic diseases and their differentiation from other autoimmune diseases.
The gold standard for the determination of ANA is the indirect immunofluorescence test (IIFT) with human epithelial cells (HEp-2), which is known for its high sensitivity and specificity. In the microscopic evaluation it is possible to establish precisely how an indicator dye (generally fluorescein) is distributed in the cells. Depending on the location of the individual autoantigens, a typical fluorescence pattern for every type of autoantibody bound will appear.
The revised international consensus on a standardised nomenclature of HEp-2 cell patterns in indirect immunofluorescence (ICAP, www.anapatterns.org) defines 15 nuclear, 9 cytoplasmic and 5 mitotic patterns which are relevant for the diagnostics of various autoimmune diseases.
Furthermore, ICAP recommends that IIFT results be confirmed by additional specific antibody tests (e.g. ELISA, line blot, ChLIA). However, the exclusive use of these monospecific test methods is insufficient for the determination of autoantibodies against cell nuclei, as not all relevant antigens are available yet in purified form. Thus, the corresponding ANAs can only be detected by IIFT.
Strategy for the Determination of ANA and Cytoplasm Components
The optimal choice for ANA diagnostics
ELISA test systems for the determination of antibodies against nuclear and cytoplasmic antigens
EUROLINE Nuclear and Cytoplasmic Antigen Profiles
EUROLINE systemic sclerosis profiles
Chemiluminescent immunoassays for rheumatic disease diagnostics
Filter techniques:
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Pharma Ethique SARL
Kouba Alger
Résidence J8, Appreval Lot C N° 169
Algeria
Phone: +213 (0) 23 60 70 33
Mobile: +213 (0) 560 97 00 02
Email: sarl.pharmaethique(at)gmail.com
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Mr. Emanuel Sá Miranda
EUROIMMUN Portugal, Unipessoal Lda.
2740-122 Porto Salvo
Taguspark
Edifício Tecnologia 2.2, 47
Portugal
Phone: +351 218 750 000
Email: geral(at)euroimmun.pt
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Ms.Gudrun Sahlmüller
EUROIMMUN Dubai Office/ Academy
Dubai
Business Bay/Bay Square Bldg.6 – 205
UAE
Phone: +971 4 3708938
Email: g.sahlmueller(at)euroimmun.de
Web: www.euroimmun.com
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Mrs. Claudia Ohst
EUROIMMUN South Africa
7441 Table View - Cape Town
5 Winton Close
South Africa
Phone: +27 21 5577666
Mobile: +27 72 6062320
Fax: +27 21 5577666
Email: c.ohst(at)euroimmun.co.za
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Heba Mekkawy
Gamma Trade Co. For Scientific Services and Consultation, S.A.E.
Cairo
14th El-Fath St., Off Shehab St., El-Mohandessen
P.O. Box: 61/12655
Egypt
Phone: +2 02 37480997 (6 lines)
Mobile: +2 0122 2223203
Email: Heba.mekkawy(at)gammatrade.com
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Mrs. Oumou Kantom Sene Faye
2Iris
Dakar-Fann
BP 15373 Point E rue 16 x 37 No 4325
Senegal
Phone: +221 33 824 37 37
Fax: +221 33 824 37 37
Email: oumou.faye(at)2iris.com
Web: www.2iris.net
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Eng. Kutaiba H. Alassaf
Technical Medical Corner - TMC
Erbil
Baghdad AL Saadon Str, 60th str.
Iraq
Phone: +964 060766993
Mobile: +964 7701755733
Fax: +964 60817002
Email: tmc_iraq(at)yahoo.com
Web: www.tmc-iraq.com
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Mr. Belal Jebriel
Agencies Med. Lab. Trading Company Ltd. (Jordan)
11185 Amman, Khalda
Otba Bin Ghazwan Street
Building no. 26 office no.304
Jordan
Phone: +9626 5548692
Mobile: +9627 88088886
Fax: +9626 5548693
Email: info(at)amt-jo.com
Web: www.amt-jo.com
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Gitiche Chege
Hospital Consumables Kenya Limited
Nairobi
Mumias South Road
GEMUWA House, office no. 23 Buru Buru Shopping Center, P.O. Box 11038-00100
Kenya
Phone: +254 202067969
Email: info(at)hckl.co.ke
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Dr. Dalal Mohammad Al Shemali
Innomedics Medical company (IMC)
13114 Safat/Kuwait
KIPCO Tower,25 th floor, Khaled Ibn El Waleed St., Shaq
P.O. Box 25363
Kuwait
Phone: +965 22215622
Fax: +965 22490959
Email: d.alshemali(at)innomedics.net
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Ets. F.A. KETTANEH S.A.
1107-2020 Beirut
Riad El-Solh
P.O. Box 11-0242
Lebanon
Phone: +9611565301
Fax: +9611565302
Email: medical(at)kettaneh.com
Web: www.kettaneh.com
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Mr. Omar Alujali
Modern Sciences Company
Benghazi
Al Jala Hospital next to Alrazi Med Lab Floor 1/ Flat 1
Libya
Phone: +218-92-498-3841
Mobile: +218 914342604
Email: omar.alujali(at)msc-libya.com
Web: www.msc.com.ly
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MENOUBIA KHECHIMI
BIOMAX MAURITANIE SARL
Nouakchott
ILOT N° 175 IMMEUBLE LALLA MEDICALE 4e ETAGE
BP 2434, SARL
Mauritania
Email: info(at)biomax-mauritanie.com
Web: www.biomax-mauritanie.com
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Mr. Mohamed Chraibi
BIOCORP SARL
20000 Casablanca
27, Rue Abou Hamed El Ghazali
Quartier Lusitania
Morocco
Phone: +212 22 48 72 16
Fax: +212 522 487994
Email: biocorp(at)biocorp.ma
Web: www.biocorp.net.ma
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Mr Emanuel Sá Miranda
EUROIMMUN Portugal, Unipessoal Lda
2720-507 Amadora
Rua Sacadura Cabral, 9 A/B
Quinta do Borel
Portugal
Phone: +351 21 4996552
Fax: +351 21 4996559
Email: geral(at)euroimmun.pt
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Yogini P. Modi
Integrated Medical Solutions L.L.C.
Muscat
Al Hamriya Postal Code 131
P.O. Box 1196
Sultanate of Oman
Phone: +968 247 07547
Fax: +968 247 07537
Email: yogini(at)imsoman.net
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Mr. Khamis Idkadek
ARCOMed.co.Ltd
91541 Salah Ideen St. Jerusalem
Al-Bireh, Sateh Marhaba, Al-Bireh - Al-quds Main Road, Al-Kholafa St., Arcomed Building
P.O.Box 54260
Israel
Phone: +972 2 2956576
Fax: +972 2 2951866
Email: arcomed(at)arcomed.ps
Web: www.arcomed.ps
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Dr. Ahmad Nassar
New Concept Pharma Trade
Doha
Office 304, 30th floor, marina twin towers
Tower B, Lusail
Qatar
Fax: 00974-44353044
Email: a.nass(at)ncpharma.net
Web: www.ncpharma.net
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Claude Audiberti
EUROIMMUN France SAS
77600 Bussy Saint Martin
Espace Villa Parc – L’Erable 1, Avenue Marne et Gondoire
France
Phone: +33 164616666
Fax: +33 164616220
Email: euroimmun(at)euroimmun.fr
Web: http://www.bio-advance.com/
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Mr. Jalal Stouhi
Samir Trading & Marketing CJSC
21421 Jeddah
Hindi Building, Madina Road Kilo 14, 599
P.O.Box 268 Al Khobar 31952
Kingdom of Saudi Arabia
Phone: +966 8942674
Fax: +966 38948162
Email: jalal.stouhi(at)samirgroup.com
Web: www.samirgroup.com
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Mrs. Claudia Ohst
EUROIMMUN South Africa (Pty) Ltd
7441 Table View - Cape Town
5 Winton Close
South Africa
Phone: +27 21 557 7666
Mobile: +27 72 606 2320
Email: c.ohst(at)euroimmun.co.za
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Dr. Khalifa Eltayeb Khalafalla
K-BIOanalytica Co. Ltd.
Khartoum
Al - Sayed Abdelrahman Street –Al-Qassr Junction
Rayan Specialized laboratory Building/ P.O.Box 11656
Sudan
Phone: +249 123 447270
Fax: +249 183 741165
Email: k_khalafalla(at)bioanalytica-rayan.com
Web: https://bioanalytica-rayan.com/euroimmun/
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Ghazi Mseddi
BMS Biologie Medecine Services
3002 Sfax
Route El Mahdia Km 1 Avenue Palestine No 76
Tunisia
Email: bms(at)gnet.tn
Web: www.bms-biologie.com.tn
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Mr. Ngattu Johnnie Mary
Chemmart Uganda Limited
Uganda
Plot 4-6 Bell close Luzira, UPET House
Behind 2nd Total Fuel Station
UGA
Phone: +256 394 845 267
Mobile: +256 782 480 969
Email: info(at)chemmartug.com
Web: www.chemmartug.com
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Khalid Abdalla Abdelrahim AL Fahim
AL Fahim Health Care Solutions LLC
Dubai
Boulevard Plaza Tower 2, Office 1904
P.O.Box 90666
United Arab Emirates
Phone: +971 4 2805852
Mobile: +971 505399422
Fax: +971 505399422
Email: info(at)alfahimhealth.com
Web: www.alfahimhealth.com
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Mr Talal Al-Shoewafi
TMA PHARMA
0967 St. Taizz
266 Jamal Abdul Nasir
Republic of Yemen
Phone: +967 773 222 444
Fax: +967 1475126
Email: Talal(at)TMApharma.com
Web: www.tmapharma.com
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