Autoantibodies against Endomysium and Gliadin

Indirect Immunofluorescence Test: EUROPLUS™ Primate Liver and Gliadin (GAF-3X) BIOCHIPs

: Primate liver and gliadin (GAF-3X) BIOCHIP: antibodies against endomysium and gliadin.

Detection of antibodies against endomysium and gliadin.
Indications: gluten-sensitive enteropathy (celiac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
Initial dilution 1 : 10; conjugate class anti-human IgA or IgG, primate absorbed, FITC-labelled.
Autoantibodies against endomysium react with many types of tissue, e.g. primate oesophagus. The most suitable substrate, however, is primate liver: in the case of a positive sample, filamentous linings of the intralobular sinusoids react.
With the gliadin (GAF-3X)-coated BIOCHIP, antibodies against gliadin can be analyzed in one and the same test procedure.
Both anti-endomysium antibodies and antibodies against gliadin (class IgA) are reliable serological markers for an active gluten-sensitive enteropathy. Therefore, their determination can in many cases replace endoscopy and biopsy.

Monospecific detection of antibodies against gliadin.
Indications: gluten-sensitive enteropathy (celiac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
Serum dilution 1 : 200; conjugate class anti-human IgA or IgG, POD-labelled.
3-point calibration. Identical incubation conditions and times: the investigation of IgA and IgG antibodies can be combined without difficulty on one and the same microplate.
Antigen: Gliadin-analogue fusion peptide (GAF-3X).
The quantitative determination of antibodies against gliadin is very suitable for monitoring the progress of the disease, compliance with a gluten-free diet, or a gluten tolerance test.

Monospecific detection of antibodies against tissue transglutaminase.
Indications: gluten-sensitive enteropathy (celiac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
Serum dilution 1 : 200; conjugate class anti-human IgA or IgG, POD-labelled.
3-point calibration, quantitative.
Antigen: recombinant, expression with the baculovirus vector in insect cells.